Why do you wake up everyday in the morning and go to sleep at night? What controls this behavior? How is it controlled? We are interested in deciphering genetic codes of biological rhythms to understand how the molecular clock machinery controls circadian biochemistry, physiology, and ultimately behavior.



Principal Investigators

Shihoko Kojima

Fellow and Assistant Professor



Kojima S, Green CB. Circadian genomics reveal a role for post-transcriptional regulation in mammals. Biochemistry. 2015;54(2):124–133.  http://dx.doi.org/10.1021%2Fbi500707c

Kojima S, Green CB. Analysis of circadian regulation of poly(A)-tail length. Methods Enzymol. 2015;551:387–403.  http://dx.doi.org/10.1016%2Fbs.mie.2014.10.021


Godwin AR, Kojima S, Green CB, Wilusz J. Kiss your tail goodbye: the role of PARN, Nocturnin, and Angel deadenylases in mRNA biology. Biochim Biophys Acta. 2013;1829(6-7):571–579.  http://dx.doi.org/10.1016%2Fj.bbagrm.2012.12.004


Kojima S, Sher-Chen EL, Green CB. Circadian control of mRNA polyadenylation dynamics regulates rhythmic protein expression. Genes Dev. 2012;26(24):2724–2736.  http://dx.doi.org/10.1101%2Fgad.208306.112


Douris N, Kojima S, Pan X, et al. Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes. Curr Biol. 2011;21(16):1347–1355.  http://dx.doi.org/10.1016%2Fj.cub.2011.07.018

Kojima S, Shingle DL, Green CB. Post-transcriptional control of circadian rhythms. J Cell Sci. 2011;124(Pt 3):311–320.  http://dx.doi.org/10.1242%2Fjcs.065771

Niu S, Shingle DL, Garbarino-Pico E, Kojima S, Gilbert M, Green CB. The circadian deadenylase Nocturnin is necessary for stabilization of the iNOS mRNA in mice. PLoS One. 2011;6(11):e26954.  http://dx.doi.org/10.1371%2Fjournal.pone.0026954


Kawai M, Green CB, Lecka-Czernik B, et al. A circadian-regulated gene, Nocturnin, promotes adipogenesis by stimulating PPAR-gamma nuclear translocation. Proc Natl Acad Sci U S A. 2010;107(23):10508–10513.  http://dx.doi.org/10.1073%2Fpnas.1000788107

Kojima S, Gatfield D, Esau CC, Green CB. MicroRNA-122 modulates the rhythmic expression profile of the circadian deadenylase Nocturnin in mouse liver. PLoS One. 2010;5(6):e11264.  http://dx.doi.org/10.1371%2Fjournal.pone.0011264


Green CB, Douris N, Kojima S, et al. Loss of Nocturnin, a circadian deadenylase, confers resistance to hepatic steatosis and diet-induced obesity. Proc Natl Acad Sci U S A. 2007;104(23):9888–9893.  http://dx.doi.org/10.1073%2Fpnas.0702448104

Kojima S, Matsumoto K, Hirose M, et al. LARK activates posttranscriptional expression of an essential mammalian clock protein, PERIOD1. Proc Natl Acad Sci U S A. 2007;104(6):1859–1864.  http://dx.doi.org/10.1073%2Fpnas.0607567104


Kojima S, Hirose M, Tokunaga K, Sakaki Y, Tei H. Structural and functional analysis of 3' untranslated region of mouse Period1 mRNA. Biochem Biophys Res Commun. 2003;301(1):1–7.  http://www.ncbi.nlm.nih.gov/pubmed/12535631


Kojima S, Yanagihara I, Kono G, et al. mkp-1 encoding mitogen-activated protein kinase phosphatase 1, a verotoxin 1 responsive gene, detected by differential display reverse transcription-PCR in Caco-2 cells. Infect Immun. 2000;68(5):2791–2796.  http://www.ncbi.nlm.nih.gov/pubmed/10768974