The separation of the chromosomes in anaphase is one of the most dramatic events of the cell cycle. It is irreversible and therefore needs to be highly accurate and tightly coordinated with mitotic exit. We combine perturbation experiments, live cell imaging, and computational modeling to understand how this high degree of coordination is achieved.
When cells divide, they need to pass on copies of the genetic information to both daughter cells. This step is controlled by a signaling pathway called the spindle assembly checkpoint. If the checkpoint fails, cells can become aneuploid, i.e., have the wrong number of chromosomes, which is a condition associated with cancer. We study how the checkpoint signaling network is constructed and which features allow it to work reliably.
Our aim is to understand basic conserved principles of cellular regulation and to be able to quantitatively explain cellular phenomena. In most of our work, we therefore combine experiments with computational modeling. This allows us to explore types of regulation that are too difficult to understand intuitively. We work with fission yeast, a simple eukaryote, which is an excellent model for eukaryotic cells in general, including human cells.
|Tatiana Boluarte||Visiting Scholar|
|Saahil Golia||Visiting Student|
|Stephan Kim||Visiting Student|