Research in the Cimini lab focuses on two major areas: (i) the role of mechanics and dynamics of mitotic apparatus components in ensuring accurate chromosome segregation during cell division; and (ii) the causes and consequences of aneuploidy (abnormal chromosome numbers) in normal and cancer cells.

Highlights

People

Principal Investigators

Daniela Cimini

Fellow and Associate Professor

Publications

2016


Cojoc G, Roscioli E, Zhang L, et al. Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore. J Cell Biol. 2016;212(7):767–776.  http://www.ncbi.nlm.nih.gov/pubmed/27002163

Guarguaglini G, Cimini D. The centrosome: a multifaceted cellular weapon against chromosome instability. Chromosome Res. 2016;24(1):1–4.  http://www.ncbi.nlm.nih.gov/pubmed/26700187

Kajtez J, Solomatina A, Novak M, et al. Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores. Nat Commun. 2016;7:10298.  http://www.ncbi.nlm.nih.gov/pubmed/26728792

Pampalona J, Roscioli E, Silkworth WT, et al. Chromosome Bridges Maintain Kinetochore-Microtubule Attachment throughout Mitosis and Rarely Break during Anaphase. PLoS One. 2016;11(1):e0147420.  http://www.ncbi.nlm.nih.gov/pubmed/26784746

Rutledge SD, Cimini D. Consequences of aneuploidy in sickness and in health. Curr Opin Cell Biol. 2016;40:41–46.  http://www.ncbi.nlm.nih.gov/pubmed/26919076

Rutledge SD, Douglas TA, Nicholson JM, et al. Selective advantage of trisomic human cells cultured in non-standard conditions. Sci Rep. 2016;6:22828.  http://www.ncbi.nlm.nih.gov/pubmed/26956415

2015


Nicholson JM, Cimini D. Link between aneuploidy and chromosome instability. Int Rev Cell Mol Biol. 2015;315:299–317.  http://www.ncbi.nlm.nih.gov/pubmed/25708466

Nicholson JM, Macedo JC, Mattingly AJ, et al. Chromosome mis-segregation and cytokinesis failure in trisomic human cells. Elife. 2015;4.  http://www.ncbi.nlm.nih.gov/pubmed/25942454

Russo A, Pacchierotti F, Cimini D, et al. Genomic instability: Crossing pathways at the origin of structural and numerical chromosome changes. Environ Mol Mutagen. 2015;56(7):563–580.  http://www.ncbi.nlm.nih.gov/pubmed/25784636

Saverot SE, Reese LM, Cimini D, Vikesland PJ, Bickford LR. Characterization of Conventional One-Step Sodium Thiosulfate Facilitated Gold Nanoparticle Synthesis. Nanoscale Res Lett. 2015;10(1):940.  http://www.ncbi.nlm.nih.gov/pubmed/26055476

Ye AA, Deretic J, Hoel CM, et al. Aurora A Kinase Contributes to a Pole-Based Error Correction Pathway. Curr Biol. 2015;25(14):1842–1851.  http://www.ncbi.nlm.nih.gov/pubmed/26166783

2014


Bakhoum SF, Silkworth WT, Nardi IK, Nicholson JM, Compton DA, Cimini D. The mitotic origin of chromosomal instability. Curr Biol. 2014;24(4):R148–9.  http://www.ncbi.nlm.nih.gov/pubmed/24556433

Civelekoglu-Scholey G, Cimini D. Modelling chromosome dynamics in mitosis: a historical perspective on models of metaphase and anaphase in eukaryotic cells. Interface Focus. 2014;4(3):20130073.  http://www.ncbi.nlm.nih.gov/pubmed/24904736

2013


Civelekoglu-Scholey G, He B, Shen M, et al. Dynamic bonds and polar ejection force distribution explain kinetochore oscillations in PtK1 cells. J Cell Biol. 2013;201(4):577–593.  http://www.ncbi.nlm.nih.gov/pubmed/23671311

Nain AS, Cimini D. MISP: The missing link between extracellular matrix and astral microtubules. Cell Cycle. 2013;12(12):1821–1822.  http://www.ncbi.nlm.nih.gov/pubmed/23759569

Nicholson JM, Cimini D. Cancer karyotypes: survival of the fittest. Front Oncol. 2013;3:148.  http://www.ncbi.nlm.nih.gov/pubmed/23760367

Park SW, Li W, Viehhauser A, et al. Cyclophilin 20-3 relays a 12-oxo-phytodienoic acid signal during stress responsive regulation of cellular redox homeostasis. Proc Natl Acad Sci U S A. 2013;110(23):9559–9564.  http://www.ncbi.nlm.nih.gov/pubmed/23671085

2012


Nicholson JM, Cimini D. Doubling the deck: Tetraploidy induces chromosome shuffling and cancer. Cell Cycle. 2012;11(18):3354–3355.  http://www.ncbi.nlm.nih.gov/pubmed/22918235

Silkworth WT, Cimini D. Transient defects of mitotic spindle geometry and chromosome segregation errors. Cell Div. 2012;7(1):19.  http://www.ncbi.nlm.nih.gov/pubmed/22883214

Silkworth WT, Nardi IK, Paul R, Mogilner A, Cimini D. Timing of centrosome separation is important for accurate chromosome segregation. Mol Biol Cell. 2012;23(3):401–411.  http://www.ncbi.nlm.nih.gov/pubmed/22130796

Wan X, Cimini D, Cameron LA, Salmon ED. The coupling between sister kinetochore directional instability and oscillations in centromere stretch in metaphase PtK1 cells. Mol Biol Cell. 2012;23(6):1035–1046.  http://www.ncbi.nlm.nih.gov/pubmed/22298429

2011


Creekmore AL, Silkworth WT, Cimini D, Jensen RV, Roberts PC, Schmelz EM. Changes in gene expression and cellular architecture in an ovarian cancer progression model. PLoS One. 2011;6(3):e17676.  http://www.ncbi.nlm.nih.gov/pubmed/21390237

Gregan J, Polakova S, Zhang L, Tolic-Norrelykke IM, Cimini D. Merotelic kinetochore attachment: causes and effects. Trends Cell Biol. 2011;21(6):374–381.  http://www.ncbi.nlm.nih.gov/pubmed/21306900

Nicholson JM, Cimini D. How mitotic errors contribute to karyotypic diversity in cancer. Adv Cancer Res. 2011;112:43–75.  http://www.ncbi.nlm.nih.gov/pubmed/21925301

2009


Paul R, Wollman R, Silkworth WT, Nardi IK, Cimini D, Mogilner A. Computer simulations predict that chromosome movements and rotations accelerate mitotic spindle assembly without compromising accuracy. Proc Natl Acad Sci U S A. 2009;106(37):15708–15713.  http://www.ncbi.nlm.nih.gov/pubmed/19717443

Sgura A, Cimini D. Telomeres: Function, Shortening and Lengthening. In: Mancini L, ed. Telomeres and Chromosome Segregation. Usa: Nova Science; 2009.  

Silkworth WT, Nardi IK, Scholl LM, Cimini D. Multipolar spindle pole coalescence is a major source of kinetochore mis-attachment and chromosome mis-segregation in cancer cells. PLoS One. 2009;4(8):e6564.  http://www.ncbi.nlm.nih.gov/pubmed/19668340

2008


Cimini D. Merotelic kinetochore orientation, aneuploidy, and cancer. Biochim Biophys Acta. 2008;1786(1):32–40.  http://www.ncbi.nlm.nih.gov/pubmed/18549824

2007


Cimini D. Detection and correction of merotelic kinetochore orientation by Aurora B and its partners. Cell Cycle. 2007;6(13):1558–1564.  http://www.ncbi.nlm.nih.gov/pubmed/17603301

2006


Cameron LA, Yang G, Cimini D, et al. Kinesin 5-independent poleward flux of kinetochore microtubules in PtK1 cells. J Cell Biol. 2006;173(2):173–179.  http://www.ncbi.nlm.nih.gov/pubmed/16636143

Cimini D, Wan X, Hirel CB, Salmon ED. Aurora kinase promotes turnover of kinetochore microtubules to reduce chromosome segregation errors. Curr Biol. 2006;16(17):1711–1718.  http://www.ncbi.nlm.nih.gov/pubmed/16950108

DeLuca JG, Gall WE, Ciferri C, Cimini D, Musacchio A, Salmon ED. Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell. 2006;127(5):969–982.  http://www.ncbi.nlm.nih.gov/pubmed/17129782

Kapoor TM, Lampson MA, Hergert P, et al. Chromosomes can congress to the metaphase plate before biorientation. Science. 2006;311(5759):388–391.  http://www.ncbi.nlm.nih.gov/pubmed/16424343

2005


Cimini D, Degrassi F. Aneuploidy: a matter of bad connections. Trends Cell Biol. 2005;15(8):442–451.  http://www.ncbi.nlm.nih.gov/pubmed/16023855

De Antoni A, Pearson CG, Cimini D, et al. The Mad1/Mad2 complex as a template for Mad2 activation in the spindle assembly checkpoint. Curr Biol. 2005;15(3):214–225.  http://www.ncbi.nlm.nih.gov/pubmed/15694304

Salmon ED, Cimini D, Cameron LA, DeLuca JG. Merotelic kinetochores in mammalian tissue cells. Philos Trans R Soc Lond B Biol Sci. 2005;360(1455):553–568.  http://www.ncbi.nlm.nih.gov/pubmed/15897180

2004


Cimini D, Cameron LA, Salmon ED. Anaphase spindle mechanics prevent mis-segregation of merotelically oriented chromosomes. Curr Biol. 2004;14(23):2149–2155.  http://www.ncbi.nlm.nih.gov/pubmed/15589159

2003


Cimini D, Mattiuzzo M, Torosantucci L, Degrassi F. Histone hyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects. Mol Biol Cell. 2003;14(9):3821–3833.  http://www.ncbi.nlm.nih.gov/pubmed/12972566

Cimini D, Moree B, Canman JC, Salmon ED. Merotelic kinetochore orientation occurs frequently during early mitosis in mammalian tissue cells and error correction is achieved by two different mechanisms. J Cell Sci. 2003;116(Pt 20):4213–4225.  http://www.ncbi.nlm.nih.gov/pubmed/12953065

2002


Cimini D, Fioravanti D, Salmon ED, Degrassi F. Merotelic kinetochore orientation versus chromosome mono-orientation in the origin of lagging chromosomes in human primary cells. J Cell Sci. 2002;115(Pt 3):507–515.  http://www.ncbi.nlm.nih.gov/pubmed/11861758

2001


Cimini D, Howell B, Maddox P, Khodjakov A, Degrassi F, Salmon ED. Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells. J Cell Biol. 2001;153(3):517–527.  http://www.ncbi.nlm.nih.gov/pubmed/11331303

1999


Carere A, Antoccia A, Cimini D, et al. Analysis of chromosome loss and non-disjunction in cytokinesis-blocked lymphocytes of 24 male subjects. Mutagenesis. 1999;14(5):491–496.  http://www.ncbi.nlm.nih.gov/pubmed/10473653

Cimini D, Tanzarella C, Degrassi F. Differences in malsegregation rates obtained by scoring ana-telophases or binucleate cells. Mutagenesis. 1999;14(6):563–568.  http://www.ncbi.nlm.nih.gov/pubmed/10567031

Pittoggi C, Renzi L, Zaccagnini G, et al. A fraction of mouse sperm chromatin is organized in nucleosomal hypersensitive domains enriched in retroposon DNA. J Cell Sci. 1999;112 ( Pt 20):3537–3548.  http://www.ncbi.nlm.nih.gov/pubmed/10504302

Rizzuto G, Gorgoni B, Cappelletti M, et al. Development of animal models for adeno-associated virus site-specific integration. J Virol. 1999;73(3):2517–2526.  http://www.ncbi.nlm.nih.gov/pubmed/9971837

1998


Carere A, Antoccia A, Cimini D, et al. Genetic effects of petroleum fuels: II. Analysis of chromosome loss and hyperploidy in peripheral lymphocytes of gasoline station attendants. Environ Mol Mutagen. 1998;32(2):130–138.  http://www.ncbi.nlm.nih.gov/pubmed/9776175

Cimini D, Fioravanti D, Tanzarella C, Degrassi F. Simultaneous inhibition of contractile ring and central spindle formation in mammalian cells treated with cytochalasin B. Chromosoma. 1998;107(6-7):479–485.  http://www.ncbi.nlm.nih.gov/pubmed/9914380

Pieroni L, Fipaldini C, Monciotti A, et al. Targeted integration of adeno-associated virus-derived plasmids in transfected human cells. Virology. 1998;249(2):249–259.  http://www.ncbi.nlm.nih.gov/pubmed/9791017

1997


Cimini D, Antoccia A, Tanzarella C, Degrassi F. Topoisomerase II inhibition in mitosis produces numerical and structural chromosomal aberrations in human fibroblasts. Cytogenet Cell Genet. 1997;76(1-2):61–67.  http://www.ncbi.nlm.nih.gov/pubmed/9154130

1996


Cimini D, Tanzarella C, Degrassi F. Effects of 5-azacytidine on the centromeric region of human fibroblasts studied by CREST staining and in situ hybridization on cytokinesis-blocked cells. Cytogenet Cell Genet. 1996;72(2-3):219–224.  http://www.ncbi.nlm.nih.gov/pubmed/8978782

Parry JM, Parry EM, Bourner R, et al. The detection and evaluation of aneugenic chemicals. Mutat Res. 1996;353(1-2):11–46.  http://www.ncbi.nlm.nih.gov/pubmed/8692188